The Girl Squad are the minions of Madame Margaret, who wield Girlifying Rifles that can turn any person or thing hit by it into a girl and or girly things. In the alternate future seen in the episode, Madame Margaret and her minions have taken over the world and turned nearly all males on the planet into girls, but this future was averted, thanks to Numbuh 4 making the Girl Squad forever non-existent.