The 12th Virginia Volunteer Infantry Regiment was an infantry regiment raised in central Virginia for service in the Confederate States Army during the American Civil War. It fought mostly with the Army of Northern Virginia. The 12th Virginia was organized at Norfolk, Virginia, in May, 1861, using the 4th Battalion Virginia Volunteers as its nucleus. Its members were from Petersburg, Richmond, Hicksford, and Norfolk. The regiment was assigned to General Mahone's and Weisiger's Brigade in the Army of Northern Virginia.