Sarutobi Ayame (猿飛 あやめ, Sarutobi Ayame), mostly referred to as Sa-chan, is a ninja from the Oniwabanshuu by training, but is currently working as an assassin. She also has a part-time job at the Kunoichi Café. Since her initial appearance, she has developed a seriously obsessive infatuation with Sakata Gintoki, to the point of even stalking him. She is severely myopic, to the point of being almost blind (cannot tell living things from inanimate objects) without her eye glasses. It is also hinted that she doesn't see well even with her eye glasses on, as she once mistook Kagura (dressed up as Gintoki at episode 97 part 2) for Gintoki himself. However, this could solely be due to her infatuation with him. She has frequently clashed with Otae for various reasons.