Cholini was the Centauri Minister of Defense during the rule of Regent Milo Virini. In late 2262, he contacted then Prime Minister Londo Mollari while he was still serving on the Advisory Board of the Interstellar Alliance at Babylon 5. Speaking for the Regent and the Centaurum, Minister Cholini voiced their concerns that the Centauri were suspected by President Sheridan and other ISA representatives of being behind the recent series of space attacks upon civilian shipping lines. Cholini urged Mollari to do what he could to assure the ISA administration that the Centauri were not to blame for the mysterious attacks, even suggest that the Narn were responsible, using ships and weapons the Centauri left behind when they ended their second occupation of the Narn homeworld.