The Making of Mystery Science Theater 3000 is the second behind-the-scenes special for Mystery Science Theater 3000; this one was made for MST3K's new home on the Sci-Fi Channel. Act One: A quick introduction sums up the show and the special, followed by the gang riffing on the opening and a short behind-the-scenes clip from their own special. The Brains sum up the premise and plot of MST3K, the kinds of movies that are selected, and a history of the show from the KTMA years, covering Joel Hodgson's departure, Mike Nelson's takeover, and Trace Beaulieu leaving. The cast members also talk about each other's talents and skills during the segment.