The Manchester and Bolton Railway was a railway in the historic county of Lancashire, England, connecting Salford to Bolton. It was built by the proprietors of the Manchester, Bolton and Bury Canal Navigation and Railway Company who had in 1831 converted from a canal company. The long railway was originally to have built upon most of the line of the canal, but it was eventually built alongside the Salford and Bolton arms of the canal. The Act of Parliament also allowed the construction of a connection to Bury, but technical constraints meant that it was never built.