The "Professor Layton VS Gyakuten Saiban" Original Soundtrack with Special Anime Film was released as a promotional CD bundled with pre-ordered copies of Professor Layton vs. Phoenix Wright: Ace Attorney in Japan, and also incldued a celluloid film strip containing select animation cels. The tracks were composed by Tomohito Nishiura, who was responsible for the music in the main Professor Layton franchise. The first and fifth tracks were newly created for the game, whilst the second and third are updated arrangements of previous Professor Layton themes. Nishiura arranged his own version of the Pursuit theme for the final track.