The 64th Virginia Mounted Infantry Regiment, consisting of troops raised in Lee, Scott, Wise and Buchanan counties in Virginia for service in the Confederate States Army during the American Civil War, served as an infantry regiment, a cavalry regiment, and a mounted infantry (dragoon) unit. It fought mostly in western Virginia and Tennessee. During April 1864, it totaled 268 effectives, but in April 1865, less than 50 disbanded.