Sometime after all their failed attempts at taking over the world in their original series, Pinky and The Brain are forced out of Acme Labs when it is torn down and turned into a Disney Store. To make matters worse, a man named Wally Faust is pursuing the mice so that he can use them in his own world domination schemes. In their attempts to escape Faust, the duo end up in a pet store, where they -- and a turtle named Mr. Shellbutt -- are purchased by Elmyra Duff. Now stuck living in Elmyra's house, Pinky and The Brain continue planning their world domination schemes -- provided, of course, they survive the distraction of their new owner.