Cholena is only seen in An American Tail: The Treasure of Manhattan Island. She is first seen in the Underground Village, where her father asks her to be the guide for Fievel Mousekewitz, Tony Toponi, Tiger and Dr. Dithering. Tony finds Cholena rather attractive and is overly enthusiastic when she volunteers to be their guide, but an angry look from Fievel sets Tony straight. Cholena says that it's an honor. Scuttlebutt becomes confused when a large female from the tribe is hugging him tightly, but Cholena says that it is merely her handmaiden, Tankho, who has volunteered to be his guide.