Pikachutwo was created by Mewtwo through cloning Ash's Pikachu's DNA and is the secondary antagonist of the first movie. He started fighting with Ash's Pikachu the minute he saw him, although Ash's Pikachu didn't like it. This happened at another time again; however, the two Pikachu became friends. Pikachutwo differs from Ash's Pikachu with the slur in his voice and the black spiked patterns on its ears. This may have been caused by Ash disrupting the cloning process when Pikachu was being cloned.