Larke has been styled a doctor, but where he received his degree is unknown. He served as rector of St. Ethelburga's Bishopsgate, London, from 30 January 1504, until his resignation in 1542; rector of Woodford, Essex from 18 January 1526, until his resignation in the following April; and rector of Chelsea, to which he was appointed by More, from 19 March 1530 until his arrest. He was indicted on 15 February 1544, with John Ireland (a priest), German Gardiner, and John Heywood. All were condemned, but Heywood recanted on the hurdle and lived to give testimony against Cranmer.