The Cambrian explosion of species refers to the geologically sudden appearance in the fossil record of the ancestors of familiar animals, starting about 542 million years ago (Mya). In addition, a similar pattern of diversification is seen in other organisms such as phytoplankton and the various colonial calcareous microfossils grouped together as calcimicrobes. The base of the Cambrian is also marked by strong geochemical perturbations, including excursions in carbon and sulfur isotopes.